Size Exclusion Chromatography
by Regina Valluzzi
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Dimensions
5.000 x 8.000 x 0.010 inches
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Title
Size Exclusion Chromatography
Artist
Regina Valluzzi
Medium
Drawing - Ink On Paper
Description
pigment ink and art marker on acid free paper, roughly 5 by 8 inches.
This is a pretty straightforward drawing of the principles in size exclusion separations and chromatography. Little porous beads are packed together, and a solution of molecules passes through the packed bed of beads. The large molecules are too big for the pores of the beads and they quickly pass straigth through the packed bed. Smaller molecules can explore more of the bead porosity and take a longer path � and longer time � through the bed. The underlying mechanism is different than, for example, paper chromatography, which you can try at home.
Uploaded
November 30th, 2011
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Comments (3)
Regina Valluzzi
I'd mentioned that you can try paper chromatography at home - it's a classic demonstration with inks that contain a mixture of dyes. You can start with ink or with a marker. If you start with a marker, dip the tip in some rubbing alcohol so you get a dilute solution of ink. If you start with ink, dilute a few drops of ink with rubbing alcohol. Have the diluted ink solution sitting at the bottom of a tall container - a jar or drinking glass. ............................................................................... Next cut a long thin strip of paper, about as wide and long as your pinky finger. Set one end of the paper in the ink solution, allowing it to get wet. Leave the rest of the paper strip standing dry outside the solution. ............................................................................... Wait for the ink solution to start crawling up the paper (through a process known as capillarity). As the ink solution crawls up the paper, you should see it start to separate into bands of color. If the experiment doesn't work on the first go, you may have to either pre-wet the paper strip with alcohol, or dilute the ink more.